Prevalence and drug resistance of extended-spectrum beta-lactamases producing Enterobacteriaceae among rural residents in villages with pig-breeding farms in Shandong province

ObjectiveTo evaluate the prevalence and antibiotic resistance of extended-spectrum beta-lactamases(ESBLs) producing Enterobacteriaceae among rural residents in villages with pig-breeding farms in Shandong province and to provide references for the control of antibiotic resistance of the pathogens.MethodsTotally 160 permanent rural residents aged ≥ 18 years were randomly selected from 4 villages with pig-breeding farms in a county in Shandong province and fecal samples were collected from the participants using eSwab sampling tube in July 2016.ESBLs-producing E.coli and K.pneumonia strains were isolated with ESBLs screening medium and identified with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS).ESBLs confirmation testing and antimicrobial susceptibility testing were performed according to Clinical Laboratory Standards Institute (CLSI) guidelines.ResultsOf the 160 fecal samples,132 were ESBLs producing bacteria positive and the carrying rate of ESBLs producing bacteria was 82.5% for the participants.Totally 207 ESBLs producing bacteria strains,including 184 ESBLs producing E.coli and 23 ESBLs producing K.pneumonia,were isolated from the 132 positive specimens.The antibiotic resistant rate for the ESBLs producing E.coli and K.pneumonia strains were 100% and 100% to cefotaxime,87.0% and 95.7% to tetracycline,82.1% and 100.0% to co-trimoxazole,64.7% and 30.4% to ciprofloxacin,56.5% and 43.5% to florfenicol,53.8%and 60.9% to gentamicin,31.0% and 78.3% to piperacillin-tazobactam,29.9% and 69.6% to fosfomycin,20.1% and 52.2% to amoxicillin-clavulanic acid,13.0% and 30.4% to nitrofurantoin,7.1% and 0 to amikacin,4.9% and 0 to cefoxitin,0 and 0 to meropenem,and 0 and 56.5% to tigecycline,respectively.Among all ESBLs producing E.coli and K.pneumonia strains,the proportions of resistant to 1-2,3-5,6-8,and ≥9 antibiotics were 7.3%,50.7%,39.6%,and 2.4%,respectively.ConclusionThe carrying rate of ESBLs producing bacteria are high and the isolated fecal ESBLs producing bacteria strains are highly resistant to cefotaxime,tetracycline,co-trimoxazole among rural adult residents in villages with pig-breeding farms in Shandong province.