Protective effect of lotus-seed milk fermented product on acute CCl₄ hepatic toxicity in mice

  Objective   To explore the protective effect and possible mechanism of Lotus-seed Milk Fer mented Product (LMFP)from acute CCl₄ hepatic toxicity in mice.

  Methods   Ascertain the effect of lotus-seed milk fermented product on LPO of normal liver homogenate and normal hepatic homogenate excitated by cysteine and ferrous sulfate in vitro.Mice were fed with distilled water, 25%, 50%, 100% LMFP and VE, intraperitoneal injected Carbon Tetrachloride(CCl₄)vegetal oil admixture in the 14th day.The examines assessing the degree of the hepaticacute damage and peroxidization were executed.Pathomorphological changes were detected by Haematoxylin eosin(HE)coloration and immunohistochemistry.

  Results   Lactiperoxidase(LPO)level of normal liver homogenate and hepatic homogenate excitated by cysteine and FeSO₄ in LMFP groups were significantly decreased compared with the control group.Compared with the model group, Hepatocyte swell, necrosis and inflammatory cells penetration in the LMFP and VE groups were relieved, Bcl-2 express enhanced.Latic dehydrogenase(LDH)activity was decreased, LPO level was observably decreased, Superoxide dismutase(SOD)activity was significantly increased in LMFP groups.(all Significance, P < 0.05).

  Conclusion   LMFP can protect liver from acute CCl₄ hepatic toxicity by the mechanism of anti-oxide system.