Study on HBsAg positive pregnant women cell transportion

  Objective   To study the route that the HBsAg positive pregnant women's cell entered fetal circulation, and to probe into the association between the cell transportion from mother to baby and HBV intrauterine infection.

  Methods   The cell transportion from mother to baby were deter mined by allele-specific PCR(AS-PCR)and heminested PCR(hemi-nPCR).The association between the cell transportion from mother to baby and HBV intratuerine infection was analyzed by noncondition Logistic regression model.

  Results   GSTM 1, ACE as maternal specific alleles: 42 mothe-rbaby pairs were recruited from hemocyte specimens as informative cases, 26(61.09%)newhorns.blood cell out of 42 informative cases had maternal cell DNA.The cell transportion from mother to baby and PBMC HBV infection in pregnant women were risk factors of HBV intrauterine infection, OR were respectively 8.40(1.42-49.74)、(OR=8.78, 1.23-62.69).

  Conclusion   s The cell transportation from HBsAg positive mothers to baby had more contribution to HBV intrauterine infection; HBV infection fetus was probably mediated by PBMC to pass through the placentae barrier and infect fetus.