Objective To determine the occurring frequency, and to clarify the clinic pathological characteristics, and to analyze the roles of EBV in the diffusion and metastasis of EBV-related gastric carcinoma in Tangshan area.The situation of EBV infection was investigated in the gastric carcinoma with different histological types in Tangshan Ares.
Methods 202 cases of formalin-fixed and paraffin-embeded tissue came from surgically resedted gastric carcinomas in Tangshan area, were confirmed further by histopathological observation by hematoxylin-eosin(HE)staining.The DNAs were extracted according to the method described by Van Beek with some modifications, and were screened for B-actin DNA detection.EBV infection was analyzed by detecting EBNA1 DNA by PCR.
Results EBVDNA were detected in 14 in 166 B-actin DNA positive cases (81.4%).14 positive cases were come from 150 adenocarcinomas(91.3%), and 5 from 15 gastric stumps(33.3%), and 0 from 16 non-adenocar cinomas(0%).
Conclusion The occurring rate of EBV-related gastric carcinoma is 8.4% in Tangshan area, which suggests that EBV infection is likely to be involved in the occurrence or development of gastric carcinoma.The infectious rate is significantly higher in gastric stumps than that in other gastric carcinomas.
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