Effect of conjugated linoleic acid on gene expression of UCP2 in obese rat fed with high fat diet

  Objective   To study the effect of conjugated linoleic acid(CLA)on expression of Uncoupling protein 2 (UCP2)in white adipose tissue of obese rats, and to explore the mechanism of reduce the body weight and fat content of obeses rats by CLA.

  Methods   Male Wistar rats were randomly separated to control group, high-fat group and high fat+CLA group(0.75 g, 1.50 g, 3.00 g% by deit weight).The effect of conjugated linoleic acid on body weight, fat content and blood lipids were observed.Using reverse transcription polymerse chain reaction(RT-PCR)technique to measure the mRNA expression level of UCP2.

  Results   CLA reduced the body weight, fat content, decreased serum triglyceride, cholesterol, free fatty acid levels, and increased the expression of UCP2 mRNA in adipose tissue of obese rat.

  Conclusion   CLA can improve dyslipidaemia of obese rat and reduce weight body and fat content by increasing the expression of UCP2 mRNA.