Study on polymorphism of chemokine receptor alleles influencing genetic susceptibility to HIV-1 infectionin

  Objective   Mutant frequency and polymorphism of HIV-1 resistance CCR-61I and SDF1-3' Aalleles were investigated in the population from Jinan Shandong province.

  Methods   The study population included 234 subjects from Jinan city.They came from Shanghe and Zhangqiu county; and none was HIV-1 positive.The genotypes of the two mutations were respectively detected by PCR/RFLP(restriction fragment length polymorphism)assay with the digestion of restriction endonuclease Bsa BI and MspI for CCR2-64I and SDF1-3' A mutation.

  Results   The frequency of the mutant alleles were: 20.25% for CCR2-64I and 21.00% for SDF1-3' in Jinan city, Shandong province.

  Conclusion   There were high frequencies of CCR2-64I and SDF1-3' Aalleles in Jinan population.The genetic susceptibility to HIV-1 infection and AIDS disease progression was low in Jinan city.