Rapid detection of drug susceptibility to antibiotics against mycobac terium tuberculosis

  Objective   To observe the prevalence stature of drug-resistance to mycobacterium tuberculosis and to provide the data of drug susceptibility for selecting effective antibiotics-Amoxicillin/potassium clavulanate.

  Methods   To collect the sputum smear-position patients in our ocal community and to get the sputum samples before the treatment with antituberculosis agents, than the samples were inoculated on Lowenstein-Jensen culture medium for isolating mycobacterium tuberculosis.The drug susecptibility test and miniumum inhibitor concentration(MICs)testing of Amoxicillin/potassium clavulaate were performed with the rapid drup susceptibility KIT.

  Results   Among the 158 test of drug susceptibility, the total drug-resistant rate to mycobacterium tuberculosis was 56.3%, 31.6%(50/158)of all samples were accorded with multidrug-resistant standards in this study.INH-and RFP-resistant rate was 8%.12% INH-, RFP-, and SM-resistance.10% for INH-, RFP-and EBresistance.4% for INH-, RFP-and PZA-resistance.26% for INH-, RFP-and PZA-resistance.61 RFP-, SM-, EB-and PZAresistance.26% for INH-, SM-EB-and PZA-resistance.6% for RFP-, SM-, EB-and PZA-resistance and 34% for INN-, RFP-, SM-, EB-and PZA-resistance.MICs of Amox icillin/potassium clavulanate with 2.0/1.0, 1.0/0.5, 0.5/0.25, 0.25/0.125 and 0.125/0.0625(μg/ml)were 2%, 2%, 22%, 58% and 16% respectively.Amoxicillin/potassium clavulanate had the significant curative effect to multi-resistant mycobacterium tuberculosis.And in the great majority of tests, at the low dose (0.25 μg/ml)so much might do the deed.

  Conclusion   Drug-resistance of the tuber culosis patients in our local community trended to multidrug-resistance at a higher level.And applying the new drug had better curative effect and made sense to direct the clinical treatment.