Effect of cholera toxoid-proinsulin conjugate and cholera toxin B sub unit admixtures on spontaneous autoimmune diabetes

  Objective   To observe the diabetes prevention effects of oral administration of human insulin and admixtures of proinsulin coupled to cholera toxin B subunit(CTB)and CTB(CTB-proinsulin+CTB)on female non-obese diabetic mice.

  Methods   40 female NOD mice were divided randomly into four groups.Group1:PBS 500Ll, group 2:insulin, group 3:CTBproinsulin 100μg+CTB 1μg, group 4:CTB-proinsulin 300 μg+CTB3 μg.The whole observation lasted 26 months.Glycosuria and glycemia were measured from 11 weeks to 26 weeks.

  Results   Oral administration of insulin and CTB-proinsulin+CTB to female NOD mice significantly suppressed incidence of diabetes.The cumulative incidence of PBS, insulin, CTB-proinsulin 100μg+CTB 1 μg, CTB-proinsulin 300 μg+CTB 3 μg were 100%, 40%, 40%, 50%(P < 0.010)respectively.Survival distribuding were all significantly difference.

  Conclusion   The needed dose of CTB-proinsulin+CTB is lower than that of insulin at the same prevention rate.