Study on HBV infection state and risk factors of HBsAg-positive pregnant women placental tissue

  Objective   To explore HBsAg-positive pregnant women placentas Hepatitis Bvirus (HBV) infection state and risk factors.

  Methods   HBeAg in pregnant women were determined by enzyme-linked immunosorbent assay (ELISA). HBsAg in placenta were detected by immunohistochemistry ABC.HBV DNA in pregnant women and their newborns were determined by nested polymerase chain reaction (nPCR). Risk factors of placenta were infected HBVwere analyzed by Logistic regression model.

  Results   41 of 205 placenta being infected by HBsAg.HBV infection rates decreased gradually from the maternal side to the fetal side(trend test χ²=9.38, P < 0.005)in the placental cell layers.Maternal decidual cells(20100%) > trophoblastic cells(18.05%) > villous mesenchymal cells(14.15%) > villous capillary endothelial cells(10.24%)Risk factors of placenta being infected HBsAg included antepartum injection hepatitis Bimmune globulin(HBIG)over thrice(OR=0.21) and HBV DNA positive in maternal serum(OR=4.77).

  Conclusion   HBVcan infect placental tissue and probably duplicate in it. Main approach of HBVinvasiving new borns via placenta is mediated by "cell transfer".Placental barrier can offer conservancy effect on fetus to some extent.HBV DNA positive in maternal serum is the risk fator of placental infection.Antepartum injection HBIG over thrice can effectively protect placenta from HBV infection and thereby decrease hidden danger of HBVinteruterine infection.