Apoptosis induced by Apoptin Gene in human lymphoma U937 Cells

  Objective   To explore apoptosis induced by apoptin gene in human lymphoma cell U937.

  Methods   The human lymphoma cell U937 was transient transformed by pcDNAA3 plasmids which contained apoptin gene, and apoptosis were measured by DNA agarose electrophoresis, RT-PCR and ELSIA at various time period.

  Results   The apoptin gene could induce apoptosis of human lymphoma cell U937 in vitro at 48 h after transformation.The mRNA of apoptin gene couldbe detected by RT-PCR at 24 h after transformation.Survial cells were associated with time after transformation.

  Conclusion   Apoptin gene can induce apoptosis in human lymphoma cell U937.