Role of mitogen activator protein kinase in genistein inhibiting vascular endothelial cell ECV304 growth facilitated by VEGF

  Objective   To explore the role of mitogen activator protein kinase (MAPK) in tumor anti-angiogenesis by genistein.

  Methods   To observe the effect of genistein on the proliferation of ECV304 cell with MTT methods. And to detect the level of the MAPK phosphorylation and the activity of the kinase in ECV304 cell with immunocytochemistry and kinase activity analysis methods.

  Results   Vascular endothelial growth factor(VEGF)alone can facilitate the proliferation of ECV304 cells and up-regulate the phosphorylation and activity of MAPK. Whereas genistein alone can cause a restraint of proliferation, down-regulate the phosphorylation and activity of MAPK(P<0.01).The growth is still restrainted when being treated with genistein and VEGF together. The level of phosphorylation and the activity of MAPK fall down compared with VEGF alone treated group and up-regulate compared with genistein alone treated group.

  Conclusion   MAPK is very important in the proliferation of ECV304 inhibited by genistein which maybe is one mechanism of anti-angiogenesis by genistein.