Objective To explore the regulation of rBD-2 gene expression by Astragalus Polysaccharides (APS).
Methods Sixty Wistar rats weighing 150 g were randomly divided into the control group and the APS group with 30 rats each. In APS group, each was dealt with APS ig; A similar dose of NS was given to the rats of the control group. Lungs were harvested on 0h, and in 1, 3, 5 and 7 d respectively after APS stimulation. Total RNA was extracted from tracheal and pulmonary tissue and rBD-2 was measured by R T-PCR.β-actin was used as internal standard.
Results The rBD-2 mRNA expressio in APS group, showed a marked increase as compared with that in the control group, and also on 3 d, compared with 1, 5 and 7 d after APS stimulation.
Conclusions Astraglus polysaccharides could up-regulate rBD-2 mRNA in rat lung tissue.
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