Study on early responses of vascular endothelial cells induced by oxidized low density lipoprotein

  Objective   To study the change of the gene expression pattern of vascular endothelial cells induced by oxidized low density lipoprotein.

  Methods   After human umbilical vein endothelial cells was exposed to oxidized low density lipoprotein(100 mg/L)for 6 hr, genes expression was screened with a complementary DNA microarray representing 4 000 different human genes and 96 control genes.

  Results   There were 3 down-regulated gene and 5 up-regulated genes.

  Conclusion   Ox-LDL could induce the change of gene expression pattern of endothelial cells. It was the first time to find that Ox-LDL could induce the change of the gene expression of H731, ST2 protein, Cyclin B1 and KDRF. These changes might be related to the dysfunction of endothelial cells induced by Ox-LDL.