Effect of glycoprotein from corbicula fluminea on apoptosis of BEL7404

  Objective   To study the antitumor effect of the glycoprotein CFp-a from corbicula fluminea against BEL7404 cancer cells in vitro.

  Methods   The inhibition rates of cancer cell was measured by MTT method.The morphological change of BEL7404 cell was detected by electronic microscopy.The effect on cell cycles of BEL7404 were observed by FCM.

  Results   The glycoprotein CFp-a had strong antitumor effect and induced apoptosis in vitro.The morphological change of apoptosis cell was also founded by electronic microscopy.A typical subdiploid peak sub G1 appeared on DNA histogram in FCM.CFp-a affected both G₁-S and G₂-M checkpoints.

  Conclusion   The glycoprotein CFp-a from corbicula fluminea can induce apoptosis of BEL7404 cell.