Protection effects of injection acanthopanax senticosus on bone marrow hematopoietic microenvironment in mice with the radiation damage

  Objective   To observe the protective effect of injection of acanthopanax senticosus on the bone marrow hemato poietic microenv ironment in mice with the radiation damage.

  Methods   (1) The model of the mice.damaged bone marr ow micrang ium was made by whole-body irradiation with ⁶⁰Coγ-radiation.(2)Mice(female)aged 6 weeks were randomly divide into the normal group, the model group, the model plus the inject ion of acanthopanax senticosus(high and low doses; 0.2 mg/10g, 0.4 ml/10g)as well as positive control group(model plus injection salvia miltiorrhiza).Mice's thigh bone marrow hemoglobin solution specimen were taken respectively to test optical density(OD)by spectrophotometry.The peripheral blood specimen were taken respectiv ely by mice-tail to test RBC, WBC and Hb quantity.To observe respectively the number of the bone marrow arteriocapilly, its area and the percentage of the capacity of hemapoictic tissue.

  Results   The injection of acanthopanax senticosus can remar kably decrease the bone marrow hemoglobin content and the number of the peripher al blood RBC, WBC and increase the number of bone marrowar teriocapillary, its area and raise the percenttage of the capacity of hemapoietic tissue in mice with the radiation damage.

  Conclusion   The injection of acanthopanax senticosus can significantly repair the bone marrow microangium and showed a protective role on the bone marrow hematopoietic micro environment in mcie with the radiaiton damage.