Study on immuno-PCR technique for early diagnosis of hemorrhagic fever with renal syndrome

  Objective   To seek a sensitive and specific method for early diagnosis of Hemorrhagic Fever with Renal Syndrome(HFRS).

  Methods   The 96 sera from patients with HFRS diagnosed by clinic were tested with immuno-PCR te-chonique for anti HF RS-IgM antibody and compared with ELISA and IFAT, and a control group(20 sera with HAV positive, 30 sera of normal human and 20 sera of HFRS IgM negative mixed with the sera of rheumatoid factor).

  Results   For the 96 sera, the positive rate with Immuno-PCR was 76% and among the 96 sera, 51 sera were from patients with febrilestag and the positive rate with the immuno-PCR was 62.7%.For the latter two methods, the positive rate were 57.3% and 39.2%, or 49% and 31.4% respectively.20 sera with HAV positive, 30 sera of nor mal human and 20 sera of HFRS IgM negative mixed with the sera of rheumatoid factor were all shown negative.

  Conclusion   The method of Immuno-PCR for detection of anti HFRS-IgM antibody was sensitive and specific, which may become a new method for early diagnosis of HFRS.