Isolation and identification of rubella virus strains from Shandong province

  Objective   For isolation and identification of rubella virus wild strain from Shandong Province.

  Methods   9 wild strains of Shandong district of rubella virus were isolated from 53 throat swabs.These virus cause marked cytopathic effect(CPE)in Vero, RK-13, BH K-21, MK2 cell lines.

  Results   9 SDRV strains were isolated.SDRV infected Vero cell showed the interference with the invasion of HSV-1, Immunofluorescence assay were used to identify the viruses, neutralization tests were used for confirmation for newly rubella virus isolations.

  Conclusion   Immuofluorescene can be the important method for rubella virus isolation and identification.