Study on resonance light-scattering of histene-DNA and its application

  Objective   To establish a sensitive, simple and safe method for the determination of nucleic acid in living body.

  Methods   In the acetic acid-sodium acetate buffer solution at pH5.5, on the basis of the measarement of the light-scattering from a complex formed by DNA and histone using a common spectrofluorometer, the DNA in mouse liver and yeast was measured.

  Results   The optimum measurement for DNA was at the wavelength of 551 nm; pH5.5;histon of 10μg/ml.The linear relationship between the light-scattering intensity(IRLS)and the concentration of nucleic acids was good(r > 0.99).The agreement between the light-scattering measurement and the conventional spectrophotometric method was satisfactory(relative error < 10%).

  Conclusion   The method was more sensitive than conventional spectrophotometic method, qaick, convenient and reliable with little interference.