Effect of benzene on CD34⁺ cell and apoptosis of mononuclear cell in bone marrow

  Objective   To study the effect of benzene on the bone marrow CD34⁺ cells number, mononuclear cell A nnexin V and F as expression of positive percentage in the workers whose lymphocyte mirokernel or percentage were higher than normal one.

  Methods   For the 20 workers and 10 normal ones, CD34⁺ cells number, mono nuclear cells Annexin V and F as was detected by flowcy to metric analysis, benzene concentration was determined by gas chromatography.

  Results   CD34⁺ cells number in group benzene concentration of 34-582 mg/m³ was significant lower than that in control group(P < 0.05), .CD34⁺ cells number was negative correlated with benzene concentration(P < 0.05), r=-0.95.Annex in V positive per-centage, expression and Fas were significanthigher than that in control group(P < 0.05).They were correlated with benzene concentration(P < 0.05), r=0.96.CD34⁺ cells number in group benzene concentration of 8-32 mg/m³ was significant higher than that in control group.

  Conclusion   Dynamic detection of CD34⁺ cells number, Annexin V and F as expression of positive percentage should be useful for early diagnosis and prevention for workers with professional benzene poison.