Effect of cooking oil fumes on cell cycle and apoptosis in lung cells of spraque dawley rats

  Objective   To detect the lung tissue cell cycle and apoptosis of spraque dawley(SD)rats after inhaling cooking oil fumes(COF).

  Methods   SD rats were used to make the chronic toxicity model.Exper iment groups were exposed to COF of three kinds of different concentration(6.88±0.31)、(15.06±0135)、(35.33±1.69)mg/m³ 171 times in 11 months, 30 min per time.Control group had inhaled clean air.Flow cytometer technique was used to detect lung tissue cell cycle and apoptosis.

  Results   Lung tissue cell apoptosis rate decreased with the rise of COF concentr at ion.There was a significant difference among different dosage groups(χ²=51.17, P < 0.01)with dose-response relationship(r=0.805, P < 0.05).G₁ cell percent decreased and S cell percent incr eased in cell cycle w ith the in COF concentration rise.There were significant difference among different dosage groups(F=5.08, P < 0.05;F=17.06, P < 0.01).

  Conclusion   G₁ cell percentage decr eased and S cell percentage increased in cell cycle with the rise of COF concentration.The lung tissue cell apoptosis rate decreased with therising in COF concentration.