Objective To investigate the methylenetetr aphydrofolate reductase(MTHFR)gene polymorphism and its interaction with plasma homocysteine, folate and vitamin B12 levels in pregnancy induced hypertension(PIH)patients.
Methods The MTHFR genotypes were determined in 82 PIH patietns(with or without hyperhomocystienemia)and 90 normal pregnant women by the PCR-RFLP method.Plasma homocysteine, folate, and vitamin B12 level were also measured.
Results The frequency of the T677/T677 genotype(0.34)in the PIH group A(with hyperhomocystienemia)was significantly higher than that in PIH group B(without hyperho mocyst ienemia)or in the control group(P < 0.01, P < 0.01 respectively), Influence of each mutant C677T allele showed a positive trend for Hcy levels but not for the fo late and vitamin B12 levels.
Conclusion Increased Hcy level in women with PIH was associated with MTHFR gene polymerphisim.The MTHFR gene T677 variant was one of the genetic risk factor for PIH.
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