Objective To analyze 351 strains isolated during 2000-2002 and related with STEC infection in order to understand their carrying mode with toxic genes.
Methods The toxic genes(stx1 and stx2), and genes of hlyA, eaeA, rfbO111 and rfbO157 of all the strains were determined with multi-PCR technology.Result 351 strains concluded 4 types of pathogens: C.freumdii, O157:H7, E.coli with rfbuisr positive but without Shigella toxic gene and ETC with rfbuisr negative but with Shiogella toxic gene.The 4 types of pathogens possessed 6 kinds of gene modes of rfbuisr, stx2 and stx1.The srains with Shigella toxic gene were mainly collected from Boer Goats, local goats and cases with diarrhea.It was also found that some livestock and poultry were infected with STEC.
Conclusion The infectios with STEC were found in Henan Province mainly O157:H7 and non-O157:H7 infection was also found.