Relationship between gene polymorphism and serum p53 protein overexpression

  Objective   To explore relationship between gene polymorphism of GSTM1, CYP1A1 and serum p53 protein overexpression.

  Methods   Case group was 127 people whose serum p53 protein was overexpression, and control group was 127 people without serum p53 protein overexpression.Polymorphism of Glutathione Stransferases M1(GSTM1)gene in peripheral leukocytes was detected by multi-differential polymerase chain reaction(MD-PCR).The polymorphism of cytochrome P4501A1(CYP1A1)gene in peripheral leukocytes was detected by allele-specific polymerase chain reaction(AS-PCR).Relationship between polymorphism of GSTM1 and CYP1A1 and serum p53 protein overexpression was evaluated by pearson Chi-Square.

  Results   When relationship between overexpression of serum p53 protein and polymorphism of CYP1A1 and GSTM1 were analysed respectively, no significant difference were found.But combined effect of GSTM1 null and CYP1A1 Val/Val increased risk in overexpression of serum p53 protein(P < 0.05, OR=3.86, 95% CI: 0.95-15.59).

  Conclusion   Combined effect of variation of GSTM1 and CYP1A1 increased risk in overexpression of serum p53 protein.