Relation between expression of ARmRNA in prostatic tissues and levels of sex hormones

  Objective   To study the effect of AR and sex hormones as well as change with age on prostatic disease.

  Methods   AR was expressed by dot blot.Sex hormones contents(testosterone、LH、cortisone、estradiol)were determined by radioimmune method.

  Results   A progressive declines of serum testosterone levels and increase of serum LH levels with age were found.Serum cortisol contents were found increased with age.Serum estradiol levels in surveyed men over 40 were higher than that of the men in control group.The estradiol concentration did not change more in the men over 50.Dot blot: AR hybrid signal of prostate cancer was stronger than that prostate hyperplasia and prostate tissues.

  Conclusion   Testosterone levels was negatively correlated with age(r=-0.45, P < 0.000 1), Chang of LH was opposite.Cortisol contents was positively correlated with age.Analysis of dot blot showed that ARmRNA expression in CaP tissues was higher than that of controls, and that ARmRNA expression in BPH tissues was no difference compared with controls.