Effect of genistein on proliferation and cell cycle of human breast cancer cell

  Objective   To investigate the influence of genistein on proliferation, cell cycle, and apoptosis of breast cancer cell MDA-MB-435S.

  Methods   Time and dose effects of genistein were detected using M TT assay.The influence of different time and dose on the cell cycle was observed by flow cytometry.The relationship of dose effects of apoptosis was observed in fluorescent microscope with acridium orange/ethidium bromide staining.

  Results   Genistein inhibited the proliferation of MDA-MB-435S cell line, the higher the dose and/or the longer the time, the more obvious the effects.Furthermore, genistein could block cell cycle progression in G₂-M, which varied with different dose and time.Genistein induced cell apoptosis, which depended on the dose.

  Conclusion   Genstein could inhibite the proliferation of human breast cancer's MDA -MB-435S cell line in vitro, the role of genistein may be the two pathways of apoptosis and cell cycle blocking.