Effect of Sagittara Sagittifolia on changes of liver lipid peroxidation and expression of c-fos mRNA induced by cadmium

  Objective   To study the effect of liver lipid peroxidation on expression of c-fos mRNA induced by cadmium.

  Methods   The male Sprague-Dawley rats were randomly assigned into six groups, each of six.The rats were pretreated intragastrically with 25%, 50%, 75% Sagittaria Sagittifolia extract(2 ml/100 g°bw), vitamin E(50 mg/100 g·bw)or distilled water once daily for ten days.Twenty-four hrs after the last dosing, animals were administered with CdCl ₂(20μmol/kg)intraperitoneally.Control animals were given distilled water only.Twenty-four hrs after the intoxication, the liver MDA, GSH content and GSH-PX activity were measured in each group above; liver c-fos mRNA was also semi-quantified by reverse-transcription polymerase chain reaction(RTPCR).

  Results   Pretreatment of rats with 5%and 75% Sagittaria Sagittifolia extract and vitamin E could decrease liver MDA content(P < 0.05), the former also increased liver GSH content(P < 0.05);However, both pretreatment of rats with Sagittaria Sagittifolia and vitamin E could not decrease liver c-fos mRNA levels induced by cadmium(P > 0.05).

  Conclusion   Lipid peroxidation seemed not or partially to involve in inducing expression of c-fos mRNA in the process of acute cadmium exposure.