Purification and Label of Artificial Sythesized Oligonucleotide

A method for purification and label of oligouncleotide wasint roduced in this paper.Oligo was synthesized with DNA assembler,isolated and purified th rough RP-HPLC and PAGE.The purified oligo was labeled with ³²P.The rest isotope was seperatedwith Sephadex G25 column.The RA of labeled oligo was over 10⁸cpm/mg DNA.The purified oligo detected with HPLC and dneatured PAGE was good for use as primer or probe.