Establishment and Application of Direct Sequencing of Hepatitis G Virus PCR Products

Direct sequencing of hepatitis G virus(HGV)PCR products was established using the ³²P end-labeled primer of PCR and Thermus aquat icus DNA polymerase(sequencing grade).By this sequencing strategy,HGV cDNA was sequenced.Clear sequence ladders were obtained and the sequence was highly identical with that of another HGV cDNA sequenced by the automatic laser fluorescent(ALF) DNA sequencer.The overlapped sequences were identical when one product was sequenced from both sides.In conclusion,direct sequencing of the PCR products can be used for nucleotide sequence analysis of HGV cDNA.