Effect of coal tar pitch smoke extracts on expression of inflammatory cytokines of THP-1 cells

Objective To explore the effect of coal tar pitch smoke extracts（ CTPE） on the exprission of inflammatory cytokines in monocyte-macrophages（ THP-1）. Methods THP-1 cells were treated with or without 2 μg / mL CTPE（ dimethylsulfoxide as vehicle） for different time（ 24,48,and 72 hours）. The expression of interleukine 1-β（ IL-1β）,interleukine 8,IL-8) and tumor necrosis factor（ TNF-α） mRNA were determined by real-time PCR. The release of TNF-αwas determined using enzyme-linked immunosorbent assay（ ELISA）. Results The expressions of TNF-α,IL-1β,and IL-8 mRNA of CTPE treatment groups were significantly different from those of the control group（ P ＜ 0. 05 for all）. The expression level of IL-1β mRNA was 2. 22 ± 0. 33 after treated with 2 μg / mL CTPE for 24 hours,while the IL-8 and TNF-α mRNA level were 4. 64 ± 3. 03 and 3. 15 ± 0. 22 for 72 hours,all of which were higher than those of control and blank groups（ P ＜ 0. 05）. The release of TNF-α for 72 hours treatment group was 538. 44 ± 53. 88 pg / mL,which was higher than that of other groups（ P ＜ 0. 05）. Conclusion CTPE could promote THP-1 cells to express TNF-α,IL-1β,and IL-8.