Abstract: Objective To assess the effects of life skill education among middle school students in counties of Shandong province,and provide basis to improve the work.Methods The cluster sampling surveys were conducted for 3 times in December of 2004,May and December of 2006.UNAIDS recommended methods were used to calculate the knowledge rate.Data analysis comprised Ch-isquare test and Chi-square trend test.Results The knowledge rates of junior school students were higher than high school students,and that of boys were higher than girls.The knowledge rates of students calculated by UNAIDS recommended methods were 151.5%,231.8%,48.3% in three sur veys separately,and were incr easing with time.Conclusion The life skills education among students achieved certain result in global fund AIDS project counties in Shandong province,but there were still imbalance between different grades and gender.