Abstract: Objective To investigate the anti-allergic effects of anthocyanin rich extract from black rice(AEBR)and to exploreits possiblem echanism.Methods The expermient of passive cutaneous anaphylax is(PCA)was used to deter mine the effect of AEBR on degranulation of mast cells in vivo.For in vitro study,various doses of AEBR were added to sensitized rat peritonealmast cells(RPMC).The IgE-induced release of degranulation was mea sured by enzymatic assay,histamine by enzyme immunoasassay(EIA),and cytokines by enzyme linked mimuno sorbent assay(ELISA).Results The treamtents of high dose of AEBR(300 mg/kg)and moderate dose of AEBR(150 mg/kg)were followed by a decrease in PCA of rats and the inhibition ratios were 49.71% and 30.40%.Whereas there sults of high dose of AEBR group (100μg/L)and moderate dose of AEBR group(50μg/L)showed significant inhibitory effect on histamine release with the inhibition ratios of 75.56% and 44.76% and the calcium uptake with the inhibition ratios 64.75% and 44.56%.Ten minutes a fter the administrations of AEBR(25,50,100μg/L),the content of tumor necrosis factor-α(TNF-α)in RPMC was decreased by 78%,80%,and 85% Ten minutes a fter the administrations of AEBR(25,50,100μg/L),the content of inter leuk in-6(IL-6)was decreased by 5.6%,77%,and 84.1% in RPMC(P<0.05).Conclusion AEBR might suppress anaphylaetic reaction through inhibition of mast cells.