广州水体中人类杯状病毒污染状况检测分析

曾爱华; 金小宝; 朱家勇

doi:10.11847/zgggws-2012-28-12-33

广州水体中人类杯状病毒污染状况检测分析

Detection of human calicivirus in different water samples from Guangzhou city

摘要

摘要: 目的调查研究广东省广州地区水体中人类杯状病毒(HuCV)的污染情况。方法收集广州地区268份自来水、715份矿泉水、119份生活污水和53份池塘水,用荧光定量PCR方法检测样本中的HuCV及GⅠ、GⅡ型的诺瓦克样病毒(NLVs)和扎幌样病毒(SLVs)。结果生活污水、池塘水、自来水、矿泉水中HuCV总检出率分别为9.24%、11.32%、0、0;生活污水中的GⅠ、GⅡ型的NLVs和SLVs检出率分别为2.52%、7.56%和0;池塘水中的GⅠ、GⅡ型的NLVs和SLVs检出率分别为1.89%、9.43%、0、1.89%;在1例生活污水中检测出GⅠ、GⅡ型NLVs,1例池塘水里同时检测出GⅡ型NLVs、SLVs。结论广州地区生活用水是安全的,但环境水体中存在着HuCV不同基因型的混合污染。

Abstract: Objective To assess the prevalence of human calicivirus(HuCV)contamination in different water samples from Guangzhou city.Methods Totally 268 tap water,715 mineral w ater,119 living sew age,and 53 pool water samples were collected.All the samples were detected with real-time fluorogenic quantitative PCR for HuCV(including GⅠ,GⅡNorw alk-like viruseNLVs,and Sapora-like virusesSLVs).Results The positive rate of HuCV for the samples was 9.24%,11.32%,0,and 0,respectively;the positive rate of GⅠNLVs,GⅡNLVs,GⅠSLVs,and GⅡSLVs for domestic sewage samples was 2.52%,7.56%,0 and 0;the positive rate of GⅠ/GⅡNLVs and GⅠ/GⅡSLVs for pool water samples was 1.89%,9.43%,0,and 1.89%,respectively.GⅠNLVs and GⅡNLVs were detected in one domestic sewage sample;GⅡNLVs and SLVs contamination were detected in one pool water sample.Conclusion The drinking water in Guangzhou area is safe,but different genotypes of HuCV contamination exists in other water samples.

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