燕麦β-葡聚糖对糖尿病大鼠肾病干预作用及机制

任锦丽; 张黎; 王思惟; 张召锋

doi:10.11847/zgggws1117131

燕麦β-葡聚糖对糖尿病大鼠肾病干预作用及机制

Intervention effect and mechanism of oat β-glucan on diabetic nephropathy in rats

摘要

摘要:
目的探讨燕麦β–葡聚糖对糖尿病大鼠肾病进展的干预作用及机制。
方法采用链脲佐菌素（65 mg/kg）一次性腹腔注射 + 单侧肾切除的方式构建糖尿病肾病大鼠模型，造模成功后随机分为4组，分别为模型组（蒸馏水），和低、中、高剂量燕麦β–葡聚糖组（0.275、0.550、1.100 g/kg），每组12只；同时另取10只大鼠行假手术并灌胃蒸馏水，为对照组；干预8周。采集血液和尿液，检测尿素氮（BUN）、尿酸（UA）、血肌酐（CR）和血清炎症因子白细胞介素–6（IL-6）、血管内皮生长因子（VEGF）水平，并取肾脏做病理组织检测。
结果与对照组比较，模型组大鼠血清尿素氮、VEGF、IL-6水平分别为（14.80 ± 3.63）mmol/L、（312.54 ± 13.39）、（145.96 ± 5.67）ng/L明显升高（P < 0.05）；与模型组比较，燕麦β–葡聚糖0.275 g/kg组大鼠血清尿素氮水平（10.39 ± 2.04）mmol/L明显下降，燕麦β–葡聚糖0.550 g/kg组大鼠血清VEGF及IL-6水平分别为（269.94 ± 16.70）、（129.71 ± 6.48）ng/L明显下降，差异有统计学意义（P < 0.05）；与模型组比较，燕麦β–葡聚糖组大鼠肾脏组织结构损伤明显减轻。
结论燕麦β–葡聚糖可有效改善糖尿病肾病大鼠肾功能，延缓肾脏组织结构损伤，其机制可能与下调炎症相关因子表达水平，改善机体炎症状态有关。

Abstract:
Objective To explore intervention effect and mechanism of oat β-glucan (OG) on diabetic nephropathy (DN) in rats.
Methods Sprague-Dawley (SD) rat DN model was established with peritoneal injection of streptozotocin(65 mg/kg·bw) plus unilateral nephrectomy. Then the model rats were divided in to 4 groups (12 in each group) : a DN model group gavaged with distilled water and three OG groups gavaged with OG at dosages of 0.275, 0.55, and 1.1 g/kg.bw once a day for 8 consecutive weeks. Another 10 normal control rats were treated with distilled water and sham operation. By the end of the experiment, blood urea nitrogen (BUN) , uric acid (UA) , and creatinine (CR) and serum vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) were measured; pathological changes of kidneys were also observed.
Results The BUN (14.80 ± 3.63 mmol/L), VEGF (312.54 ± 13.39 ng/L), and IL-6 (145.96 ± 5.67 ng/L) of the DN model rats were significantly higher than those of the normal control rats (P < 0.05 for all). Compared with those of the DN model rats, the BUN (10.39 ± 2.04 mmol/L) of the DN rats treated with 0.275 g/kg.bw OG decreased significantly; the VEGF (269.94 ± 16.70 ng/L) and IL-6 (129.71 ± 6.48 ng/L) of the DN rats treated with 0.550 g/kg.bw OG were reduced significantly (both P < 0.05). In addition, alleviated structure damage of kidney tissues were observed in all the DN rats treated with OG in contrast to the DN model rats.
Conclusion Oat β-glucan could effectively improve renal function and attenuate damages of kidney tissue by down-regulating inflammation-related factors in rats with diabetic nephropathy.

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