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王丽, 王玲, 郭辉杰, 刘志华, 刘优招, 陈清, 胡静. 不同宿主来源香港海鸥菌耐药表型和耐药基因分析[J]. 中国公共卫生, 2018, 34(6): 908-912. DOI: 10.11847/zgggws1117966
引用本文: 王丽, 王玲, 郭辉杰, 刘志华, 刘优招, 陈清, 胡静. 不同宿主来源香港海鸥菌耐药表型和耐药基因分析[J]. 中国公共卫生, 2018, 34(6): 908-912. DOI: 10.11847/zgggws1117966
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Li WANG, Ling WANG, Hui-jie GUO, . Antibiotic-resistant phenotypes and genotypes of Laribacter hongkongensis isolated from different hosts[J]. Chinese Journal of Public Health, 2018, 34(6): 908-912. DOI: 10.11847/zgggws1117966
Citation: Li WANG, Ling WANG, Hui-jie GUO, . Antibiotic-resistant phenotypes and genotypes of Laribacter hongkongensis isolated from different hosts[J]. Chinese Journal of Public Health, 2018, 34(6): 908-912. DOI: 10.11847/zgggws1117966
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不同宿主来源香港海鸥菌耐药表型和耐药基因分析

Antibiotic-resistant phenotypes and genotypes of Laribacter hongkongensis isolated from different hosts

    摘要
  • 摘要:
      目的  了解不同宿主来源香港海鸥菌的耐药表型及耐药基因的携带情况。
      方法  于2015年1月—2016年8月分离自广东省淡水产品鱼和蛙肠道117株香港海鸥菌，5株病人粪便分离株为本实验室保存。采用K-B纸片法测定菌株对13种抗生素的耐药表型，PCR法扩增磺胺类、四环素类、喹诺酮类和红霉素类等9种耐药基因，应用χ2检验进行率的差异性比较。
      结果  122株香港海鸥菌主要对利福平、头孢噻吩、氨苄西林、复方新诺明、红霉素、四环素、环丙沙星耐药，耐药率分别为86.9 %、78.6 %、48.7 %、30.8 %、18.9 %、18.0 %、14.5 %，多重耐药率达57.4％；对四环素耐药的22株菌中，tetA、tetR检出率分别为72.7 %、68.2 %；对磺胺类耐药的36株菌中，sul1、sul2检出率分别为86.1 %、50.0 %；鱼源株对氨苄西林、头孢噻吩、红霉素、利福平的耐药率均高于蛙源株（P均 < 0.05），而蛙源株对四环素、环丙沙星、复方新诺明和链霉素的耐药率均高于鱼源株（P均 < 0.05）；蛙源株的sul1、sul2，tetA、tetR、aac（6′）-ib和ereA等6种耐药基因携带率均高于鱼源株（P均 < 0.05）；5株人源株耐药率较低，仅有1株表现为多重耐药。
      结论  广东省淡水产品中香港海鸥菌对抗生素的耐药性较为严重，不同宿主来源的菌株具有不同的耐药模式。tetA、tetR和sul1、sul2可能是该菌对磺胺类和四环素类药物耐药的主要原因。

     

    Abstract:
      Objective  To examine phenotype and genotype distribution of antibiotic-resistant Laribacter hongkongensis (LH) strains isolated from different hosts.
      Methods  Totally 117 LH strains were isolated from intestinal tracts of fresh water fish and frogs in Guangdong province from January 2015 to August 2016, and 5 strains from human faces. Kirby-Bauer (K-B) disk diffusion method was used to test the susceptibility to 13 antibiotics of the strains. Detections of 9 antibiotic-resistant genes were performed for 122 LH strains with PCR amplification. Chi-square test was adopted to analyze differences in antibiotic resistance of the strains.
      Results  Of the 122 LH strains, 86.89% were resistant to rifampin, 78.6% to cefalotin, 48.7% to ampicillin, 78.6 % to sulfamethoxazole, 18.9% to erythromycin, 18.0% to tetracycline, and 14.5% to ciprofloxacin, respectively, with an overall multiple antibiotic-resistant rate of 57.4%. Among the 22 tetracycline-resistant LH strains, the detection rates of tetA and tetR resistance-related genes were 72.7% and 68.2 %; for the 36 sulfa-resistant LH strains, the detection rates of sul1 and sul2 resistance-related genes were 86.1% and 50.0%. The ampicillin-, cefalotin-, erythromycin-, and rifampicin-resistant rate were significantly higher among LH strains isolated from fresh water fish than among those from frogs (P < 0.05 for all); while the tetracycline-, ciprofloxacin-, sulfamethoxazole-, and streptomycin-resistant rate of the LH strains from frogs were significantly higher than those of the strains from fish (P < 0.05 for all). Significantly higher carrying rates of antibiotic resistance-related genes, including sul1, sul2, tetA, tetR, aac (6′) -ib, and ereA, were observed among the LH strains isolated from frogs than among those from fish (all P < 0.05）. Of the 5 LH strains from human, only one was multiple antibiotic-resistant.
      Conclusion  The antibiotic-resistant stains are highly prevalent for LH isolated from freshwater products in Guangdong province and the antibiotic resistance is different for the LH strains isolated from various hosts. Carrying tetA, tetR, sul1, and sul2 genes may play a main role for the LH strains′ sulfonamides and tetracyclines resistance.

     

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