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宗莉, 刘学升, 毛玲玲, 孙英伟, 王子江, 孙思浓, 刘芸, 张洁, 赵卓. 辽宁省2011 — 2017年发热伴血小板减少综合征流行病学特征与M片段基因序列分析[J]. 中国公共卫生, 2019, 35(5): 644-647. DOI: 10.11847/zgggws1121621
引用本文: 宗莉, 刘学升, 毛玲玲, 孙英伟, 王子江, 孙思浓, 刘芸, 张洁, 赵卓. 辽宁省2011 — 2017年发热伴血小板减少综合征流行病学特征与M片段基因序列分析[J]. 中国公共卫生, 2019, 35(5): 644-647. DOI: 10.11847/zgggws1121621
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Li ZONG, Xue-sheng LIU, Ling-ling MAO, . Prevalence and M-fragment gene sequencing of fever with thrombocytopenia syndrome virus in Liaoning province, 2011 – 2017[J]. Chinese Journal of Public Health, 2019, 35(5): 644-647. DOI: 10.11847/zgggws1121621
Citation: Li ZONG, Xue-sheng LIU, Ling-ling MAO, . Prevalence and M-fragment gene sequencing of fever with thrombocytopenia syndrome virus in Liaoning province, 2011 – 2017[J]. Chinese Journal of Public Health, 2019, 35(5): 644-647. DOI: 10.11847/zgggws1121621
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辽宁省2011 — 2017年发热伴血小板减少综合征流行病学特征与M片段基因序列分析

Prevalence and M-fragment gene sequencing of fever with thrombocytopenia syndrome virus in Liaoning province, 2011 – 2017

    摘要
  • 摘要:
    目的 了解辽宁省2011 — 2017年发热伴血小板减少综合征（SFTS）的流行病学特征及病毒分离株的基因型分布和进化规律，为SFTS的诊断和制定预防控制策略和措施提供参考依据。
    方法 以辽宁省2011年1月 — 2017年12月SFTS确诊病例为研究对象，描述疾病的流行病学特征；将病原体按年份进行分层随机抽样，每年随机选取5株，采用RT-PCR方法扩增分离株M片段基因序列，应用软件Mega 6.0软件进行同源性分析，构建亲缘进化树。
    结果 辽宁省2011年1月 — 2017年12月共报告SFTS确诊病例438例，死亡病例19例，病死率为4.34 %；发病时间集中于春夏季，每年7 — 9月为发病高峰；省内高发地区为丹东市（242例）和大连市（144例）；主要发病人群集中于 ≥ 45岁中老年人群，占总报告病例数的89.95 %；农民及家务待业者发病较多，占总报告病例数的91.32 %。基因序列分析结果显示，辽宁省35株发热伴血小板减少综合征布尼亚病毒（SFTSV）分离株的核苷酸序列同源性分别为92.20 %～100 %，且在C和J2个基因型上均有分布，25株分布在C2基因型分支。
    结论 辽宁省SFTS发病具有一定的季节性和地域性，≥ 45岁的中老年农民为高危人群；2011 — 2017年辽宁省SFTSV流行优势株为C2基因亚型。

     

    Abstract:
    Objective To examine prevalence characteristics of severe fever with thrombocytopenia syndrome (SFTS) and to analyze genetic distribution and evolution of SFTS virus (SFTSV) in Liaoning province during 2011 – 2017.
    Methods We collected the data on all confirmed SFTS cases reported in Liaoning province from January 2011 through 2017 and described the prevalent characteristics of the cases. Five SFTSV strains were randomly selected from all the isolated strains each year and the M-fragment gene sequence of the strains was amplified with reverse transcription-polymerase chain reaction (RT-PCR) and homology analysis was performed using software MEGA 6.0 to construct the relative evolution tree.
    Results Totally 438 confirmed SFTS cases were reported in the province during the period; of them, 19 were dead, with a case fatality rate of 4.34%, and 89.95% were aged ≥ 45 years. More cases were reported during the spring and summer season (July to September in a year) and more cases (242 and 144) were reported in Dandong and Dalian municipality. The most (91.32%) of the reported cases were farmers, house workers and unemployed people. The sequence analysis revealed that the nucleotide sequence homology for the 35 isolated SFTSV strains was 92.20% – 100%; both C and J genotypes were observed among the 35 strains and 25 strains were of C2 genomic branch.
    Conclusion In Liaoning province between 2011 – 2017, SFTS epidemic showed seasonal and regional distribution; farmers aged 45 years had a higher risk of SFTS; and SFTSV of C genomic subtype was the prevalent strain.

     

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