康强硒、硒多糖对染砷大鼠肝细胞增殖周期的影响

郑金平; 祝寿芬; 窦岩

doi:10.11847/zgggws2000-16-11-24

康强硒、硒多糖对染砷大鼠肝细胞增殖周期的影响

The Effect of Kangqiang Selenium and Selenium Polysaccharide on Cell Cycle of Livers of Rats Poisoned with Arsenic

Zheng Jinping,

摘要

摘要: 选用Wistar大鼠随机分为对照组(蒸馏水)、砷组(4.6mg/kgAs₂O₃)、砷+康强硒①组(4.6mg/kg+0.1mgSe³⁺/kg)、砷+康强硒②组(4.6mg/kg+0.2mgSe³⁺/kg)、砷+硒多糖组(4.6mg/kg+40mg/kg)5组.流式细胞仪测定肝细胞周期,病理组织切片观察病理改变.结果染砷组大鼠肝细胞比对照组G₀/G₁期细胞数显着减少,S和G₂+M期细胞数显着增多,细胞DNA相对含量(DNARC)及细胞增殖指数(PI)均显着增加,显示DNA合成增加,同时,部分细胞在G₂+M期阻滞.康强硒、硒多糖等硒制剂可有效拮抗砷对肝脏细胞增殖周期的影响.含硒0.2mgSe³⁺/kg体重的康强硒效果优于含硒0.1mgSe³⁺/kg体重的康强硒,与硒多糖相当.表明一定剂量的砷可引起肝脏细胞DNA合成增加,G₂+M期阻滞,导致细胞增殖周期紊乱,与砷中毒引起的肝脏损伤及肝肿瘤的发生有关.康强硒及硒多糖可有效拮抗砷的这种毒作用.

Abstract: ntagonism of Kangqiang Selenium and Selenium Polysaccharide against arsenic.The cell cycle was observed with the flow cytometer.The percentage of cell in G₀/G₁ phase was decreased,but the percentage of cell in S and G₂+M phases and the proliferation index(IP)、the DNA relate content(DNARC)were increase greatly,which shows that the production of DNA increased and the cell were arrested in G₂+M phases.The selenium compounds (Kangqiang Selenium and Selenium Polysaccharide)could protect against the effects of arsenic on livers cell cycle in rats.The antagonism of Kangqiang Selenium with 0.2mgSe³⁺/kg was stronger than it with 0.1mgSe³⁺/kg and same as Selenium Polysaccha2 ride.It shows that arsenic could interfere with the liver cell cycle. which was related to the liver cancer induced by arsenic.Kangqing Selenium and Selenium Polysaccharide could actively protect against the effects of arsenic.

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