转移因子对甲肝疫苗的热稳定性及免疫佐剂作用

The Function of Tranfer Factor to the Stability and Adjuvant of Attenuated Live Vaccine of HAV

摘要: 转移因子(TF)具有增强甲肝疫苗热稳定性的作用,其效果与1M的MgCl₂相当,加入TF的甲肝疫苗在4～8℃保存6个月、室温保存21天、37℃保存7天,滴度基本不变,仍在6.5LgCCID₅₀/ml的合格范围内.当提高保存温度时,这种作用效果更明显;另外,TF还具有一定的免疫佐剂作用,甲肝疫苗与TF混合后免疫小白鼠,阳转率(10/10)比对照组(8/10)高,几何平均滴度是对照组的1.63倍.

Abstract: The study is about the transfer factor(TF)being capable of enhancing the stability of attenuated live vaccine of HAV with the effect as same as 1M MgCl₂.The titer of vaccine mixed with TF still was higher than 6.50 log CCID/ml after being stored at 4～8℃ for 6 months,room temperature for 3 weeks.37℃ for 7 days. The result showed that the HAV antibody rates of the mice immunized by live attenuated Hepatitis A vaccine with or without TF were 100% and 80% after injected for 5 weeks,and the GMT were 1:1.62 and 1:2.64.It showed good immunoresponse when live attenuated hepatitis A vaccine mix with TF.