特异血清吸附模板聚合酶链反应检测肠侵袭性大肠杆菌的研究

Studies on Detection Enteroinvasive E.coli By Specific Serosorbernt Template Polymerase Chain Reaction

摘要: 目的建立检测与鉴定肠侵袭性大肠杆菌的新方法.方法应用特异血清吸附模板聚合酶链反应(SSST.PCR)检测肠侵袭性大肠杆菌.结果与经典的分离培养法比较,此方法大大缩短了时间,由原来的7天左右缩短到了6～8小时;与单纯的PCR法比较,简化了DNA提纯的过程,并能进行分型.结论 SSST.PCR法是一种简单、快速、特异和敏感的新方法.

Abstract: Objective To set up a new method of detecting E.coli.Methods To use specific ser osorbernt templat polymerase chain reaction(SSST.PCR)to detect Enteroinvasive E.coli.Results SSST.PCR was finished in about 6-8 hours,although bacterium clturing test was finished in about 7 days or so.Compared to pure PCR,SSST.PCR simplify purification of DNA and classify the Enter oinvasive E.coli.Conclusion SSST.PCR was rapid,easy,specific,sensitive and accurate method in detection of Enteroinvasive E.coli.