1株中国人HCV-2a型基因组部分cDNA序列的种系发生树分析

Phylogenetic Analysis of Partial cDNA Sequence of Hepatitis C Virus Derived from a Patient with HCV Infectinn in China

摘要: 用反转录套式PCR(RT-PCR)法从陕西地区1例NANB型肝炎患者血清中扩增出部分E区的cDNA片段,克隆于PGEM-T载体中用全自动序列分析仪进行双向测序,测得的序列以及已发表的同一分离株5'NRC、C区序列,分别同Genbank中注册的36株已知基因型的HCV相应区段的序列比较,构建种系发生树.结果 5'NCR区相应序列构建的种系发生树表明该分离株为2型,而用C区和E区构建的种系发生树均表明该分离株为2a型.提示该分离株为2a型;对同一病原体来说,其基因区段的变异度不同,则种系发生树分析的结果不完全相同,变异度大的结果较准确.

Abstract: Objective To clone and sequence partial cDNA of Hepatitis C virus(HCV)genome of Shanxi China,constructed phylogenetic tree.Methods A 878bp fragment in envelope region was amplified by reverse transcr iption nested polymerize chain reaction(RT-PCR).The fragment was cloned into PGEMT-vector,then sequenced by auto-sequencing instrument.Phylogenetic tree were constructed from the 5'NCR sequene of 252bp,C sequence of 436bp and E sequence of 878bp by HCV-XA and other 36 strains registered in Genbank respectively.Results Phylogenetic tree constructed from the 5'NCR indicates HCV-XA belonged to type 2;from C and Eindicate it belonged to subtype 2a.Conclusion The isolated strain belonged to type 2a.The variation of the subgenome affected the result of phylogenetic analyses for the same pathogen.