Abstract: Objective In order to investigate infection status about HBV and HCV in family members of HCC and to reveal the opportunity exposed to HBV and HCV infection.Methods HBsAg,anti-HBs,HBeAg,anti-HBe,anti-HBc,anti-HCV were detected in the sera of family members from case and control using ELISA,and HBV DNA with PCR.Results HBsAg,anti-HBe,anti-HBc and HBV total infection ratio were higher in the children of case group than that in control;anti-HBc and HBV total infection ratio in the spouse of case group were also higher than that in control.Statistical variations were significant(P<0.05 or P<0.01).The examination of HBVDN A in the children of case group was higher than that in control(P<0.05).There was a close connection between HBeAg and HBV DNA.Positive HBV DNA were with positive sera of HBeAg.There was no statistical variation between case and control in anti-HCV(P>0.05).HBsAg and anti-HBc were led into conditional logistic regression equation with odds ratio value of 27.96,4.77 respectively.Conclusion There was a trend of clustering of HBV infection in families of HCC.HBsAg and anti-HBc were representitive mar kers in Hepatitis B virus infection in the family members of HCC.Close living contact was also one of the routes of transmission.HCV infection wasn't the major type of infection.