小鼠肾脏中金属硫蛋白基因诱导表达的时程变化

Time-Course Changes of Metallothionein Gene Expression Induced by Cadmium Metallothionein in Mice’s Kidney

摘要: 目的探讨重复性注射CdMT诱导小鼠肾脏MT基因表达的时程变化.方法采用RT-PCR方法半定量检测重复性短时间间隔皮下注射镉金属硫蛋白(CdMT)染毒后小鼠肾皮质金属硫蛋白(MT1、MT2)基因表达的时程变化.结果自CdMT染毒后12h,与对照组相比肾皮质MT1基因表达有增强的趋势,染毒后12h及24h,肾皮质MT2基因表达明显增强,其表达高峰均出现在CdMT染毒后24h,至168hMT1与MT2基因表达均恢复到基准水平.结论重复性短时间间隔皮下注射CdMT能够诱导小鼠肾皮质MT1及MT2基因的表达,并且MT1及MT2基因表达的时程变化基本一致.

Abstract: ObjectiveTime-course of gene expression of metallothionein(MT1 and MT2)in kidney conex from mice were measured.MethodsFollowing an initial high CdMT dose and four subsequent low doses(50+4×25μg/kg)administered subcutaneously at an interval of 2h,with semi-quantitative R T-PCR.ResultsThe MTI gene expression level was higher at 12 h and returned its basal level at 168h after first injection.The MT2 gene expression level was significantly higher at 12 and 24h and also returned its basal level at 168h.The MT1 and MT2 gene expression level reached to the maximum at 24h.ConclusionMT1 and MT2 genes in mice's kidney cortex from mice could be induced by multiple short-interval CdMT injections.Time course of MT1 and MT2 gene expression was parallel.