Abstract: Objective To observe the acquired immunodeficiency syndrome virus exomembrane protein,core protein and Interferon(HIV-lenv/IF NA-2b,HIV-lgag/IF NA-2b)fusiongenes expression product for gene vaccine,It induces humoral immunity and cellular immunity of organism.Methods The mice are immunized using the purified recombinant virus vJ16env/IF NA-2b,vJ38gag/IF NA-2b,as control with NaCl and vaccine.The ConA,Lps's response of T-ly mphocytes in blood and spleen are examined by MTT 570nm.OD value of HIV-lenv and HIV-lg agantibody is determined.CD₄⁺,CD₈⁺ T cell figure of mice spleen is examined by Flow Cytomeuy(FCM)Results There is significant change between test group and the control for ConA,Lps's response of T-lymphocytes in blood and spleen(P<0.05).vJl6env/IF NA-2b and vJ38gag/ IFNA-2b could stimulate mice body to pro duce high level antibody(P<0.05).CD4+ T-lymphocytes sig nificantly change between test group and the control(P<0.015).CD₈⁺ T-lymphocytes do not significantly change between test group and the contral,but CD₈⁺ T-lymphocy tes have the trend to increase.Conclusion Recombinant vaccinia virus could induce mice to producte humoral immunity and cellular immunity and could response HIV-lenv,HIV-lgag positive serum.It possesses immungenicity and immunereposibility.