金属硫蛋白对甲基汞致小鼠生殖细胞损伤的拮抗作用

Resistance Effects of Metallothionein on Damage of Germ Cells Caused by Methymercury

摘要: 目的探讨ZnMT对甲基汞所致生殖细胞损伤的拮抗作用。方法采用支持细胞/生殖细胞体外共培养法观察甲基汞对生殖细胞存活率的影响以及ZnMT的拮抗效应。结果 0.01mmol/L和0.1mmol/L两组甲基汞使生殖细胞存活率明显下降,30μg/ml和50μg/mlZnMT使0.01mmol/L甲基汞作用后的生殖细胞死亡数减少,但对0.01mmol/L甲基汞作用后的生殖细胞却无此作用。结论 ZnMT对一定剂量范围的甲基汞致生殖细胞损伤有拮抗作用。

Abstract: Objective To study the resistance effects of metallothionein(ZnMT)on germ cell damage caused by methymercury(MeHg).Methods Mixed cultures of sertili and germ cells were used to observe the effects of ZnMT on the viability of germ cells of the mice treated by MeHg.Results The germ cells viability was decreased in 0.01mmol/L and 0.1mmol/L MeHg group.Concentration of 30μg/ml and 50μg/ml ZnMT could resist the decrease of viability of germ cells induced by 0.01mmol/L mercury treatment,but could not reduce the death rate of germ cells caused by 0.1mmol/L MeHg.Conclusion It indicated that ZnMT could resist the damage of germ cells to certain extent caused by mercury treatment.