Abstract:
Objective To develop a sensitive,specific,rapid method for detection and identification of human influenza viruses.
Methods RT-PCR and multiplex RT-PCR were used to detect HA1,HA3,and HA(B) genes with several type or subtype specific primers in human influenza viruses.
Results HA1,HA3,and HA(B) genes were able to be amplified by the RT-PCR and multiplex RT-PCR from human influenza viruses HA1,HA3,and HA(B) respectively.Virus types or subtypes were determined according to the length of amplified products,and the results were consistent with ones by hemagglutinaiton inhibition test.From 3 throat swabs samples of patients with upper respiratory tract infections,one was found HA3 gene posirive by the multiplex RT-PCR,which was testified by conventional chicken embryo culture and hemagglutination inhibition test.
Conclusion The RT-PCR and multiplex RT-PCR can be used in rapid detetion and identification of common human influenza viruses,and the latter is more effective.