Abstract: ObjectiveTo study the isolation,characteristics and comparison of several detection methods of Helicobacter pylori.MethodsAnaerobic cultivation and PCR were used to isolate and determine H.Pylori preliminarily.One strain was further determined by various tests of characteristics.ResultsAbout forty strains of H.pylori were isolated and preliminarily identified from gastric mucosa biopsy specimens in 106 patients presenting for gastroscopy athsopitals.HP-WGM was gram-negative,curved bacilli which ha smooth coats,several sheathed single polar flagella.After 2～3 days'incubation,it grew evidently as about 1mm in diameter,round,protruding,uniform in edge,semi-translucent coloies on urea agar rolled-tubes incubated at 37℃ under microaerophilic condition.It was positive in urease,catalase,oxydase tests and negative in H₂S producing and gelatin liquefaction tests.It couldn't reduce NO₃^-,could tolerate 3.5% NaCl and 1% glycine and hydrolyze starch.The organism is immunogenic.ConclusionHP-WGM could be used as an antigen to carry out ELISA for determining the extent of H.pylori infection.At least over two methods are necessary to diagnose the infection.But in scientific research,the culture and determination of bacteria is the most reliable way.