Abstract: Objective To establish the method for determination of biological essential trace-elements Cu,Fe,Mn,Zn in fetal cerebellum.Methods Closed-vessel microwave digestion with HNO₃+H₂O₂ was used for sample preparation and the digested analyze was directly analyzed for the determination of Cu,Fe,Mn,Zn by ICP-AES.Results The detection limit of each element was between 0.000 7mg/L and 0.03mg/L.Standard linear correlation r≥0.999.Standard deviations were below 2%.Recoveries of spiked natural samples were of the range 93%-117%.Two standard reference materials namely "Pig Liver GBW08551" and "Human Hair GBW07601" were used as quality control standards and the analysis results conformed well to the certified values.The frequency of results for Cu and Mn showed normal distribution,and the frequency of results for Fe and Zn showed skewness distribution.Conclusion The proposed method was proved to be simple,rapid,accurate and reliable,and ithas been applied to the analysis of the samples with satisfactory results.