Abstract: Objective To explore the charaeteristics of infection and drug resistance of nonfermentative Gram-negative bacilli and to guide the proper use of antibioties,and then to reduee the production of drug resistance stains in intective patients.Methods 666 strains of nonfermentative eram-negative baeilli from kinds of clinical specimen during 1999 Jan-2001june were analyzed retrospeetively.Results 666 strains of nonfermentative Gram-negative bacilli were separated out from the 4272 pathogenic stains(15.60%),and the rate of the drug resistanee to primary eephalosporis was approximate to 90.94%;and the rate of the drug resistanee to amoxyeillin and aztreonam attained 86.88% and 44.38% respectively.Conclusion The pereentage of nonfermentative Gram-negative baeilli separated out from positive stains is higher,and the drug resistanee to frequently-used antibiotics of nonfermentative Gram-negative baeilli is higher,we should lay emphasis on choosing correctly the antibiotics.