Abstract:
Objective To investigate the role of antioxidants-catalase,reduced glutathione and β-carotin in UVR-induced DNA damage.
Methods After extracting human peripherial blood lymphocytes and treating with catalase,reduced glutathioneβ,-carotin as well as ultraviolet radiation,the damaged degree of lymphyocytic DNA was examined with single cell gel electrophoresis.
Results With the UVR dose increasing,the damaged degree of lymphocytic DNA increase gradully;after treating with three antioxidants UVR-induced DNA damage was reduced and its effects showed dose-dependent manner within a certain range.
Conclusions Catalase,reduced glutathione andβ-carotin were perfect antagonists to UVR-induced DNA damage;single cell gel electrophoresis was an ideal method of analyzing UVR-induced DNA damage.