肾综合征出血热病毒基因型和流行特点分析
Analysis on genotype an epidemic characteristics of hemorrhagic fever with renal syndrome virus
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摘要:目的 探讨山东省汉坦病毒(HV)主要流行株的基因型和流行特点.方法 采集山东地区有代表性的鼠肺标本, 应用逆转录聚合酶链反应(RT-PCR)扩增技术及核苷酸序列测定方法对免疫荧光抗原阳性的标本进行HVG2基因扩增并测序, 与原有山东毒株一起进行同源性分析、系统发生树分析.结果 山东省新分离18株HV均为汉城(SEO)型, 与以往山东SEO型HV有较高同源性, 在95.7% 以上, 属于同一亚型.山东省汉滩(HTN)型HV同源性较低, 在77.9% 以上, 属于不同亚型.结论 山东省HV是以SEO型为主的SEO和HTN混合型疫区.SEO型基因变异率低, 有较高的稳定性.而HTN型HV变异率高, 稳定性差.Abstract:Objective To understand the genetic type of Hantaviruses(HV)and the differences betw een the Msegments isolated from rodents in Shandong Province.Methods The lung samples positive with HV antigens by immunofluorescent as say were amplified by RTPCR with Hantavirus specific primers and the products sequenced.The results were compared with the previous strains of Shandong Province in homolog y and phylogenetics.Results Eighteen of the PCR amplifications were positive.Nucleotide sequence homology analysis showed that they were all seoul(SEO)-type HV.The nucleotide sequence homology of these samples was more than 95.7% and they belonged to the same subtype.The nucleotide sequence homology of the Hantaan(HTN)type HV in Shandong Province was more than 77.9%, but they belonged to different subtypes.Conclusion The HV strains isolated in Shandong Province mainly were SEO type.The SEO type HV was highly conserved while the HTN type HV was highly variable.
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