GbE对TCE诱导人KC细胞氧化损伤的影响
Protective effects of extracts from leaves of Ginkgo biloba on TCE-induced oxidation insult in human keratinocytes
-
摘要:目的 探讨三氯乙烯(Trichloroethylene, TCE)对人角质形成细胞(Keratinocyte, KC)氧化损伤的影响及银杏叶提取物(Extracts from the Leaves of Ginkgobiloba, GbE)对其拮抗作用。方法 采用体外KC无血清培养方法, 用中性红吸收实验(Netrual Red Uptake, NRU)测定TCE对KC细胞毒性的半数抑制浓度NR50;用乳酸脱氢酶(LDH)法检测KC的损伤; 用硫代巴比妥酸法测定KC细胞脂质过氧化物丙二醛(MDA)活力; 同时检测细胞内抗氧化酶超氧化物歧化酶(SOD)的活力。结果 TCE对KC的NR50为4.53mmol/L; KC在TCE(0.125, 0.25, 0.5, 1.0, 2.0mmol/L)作用1, 2, 3, 4h后, LDH释放增加, 且具有时间和剂量依赖性, KC在TCE作用4h后, MDA的生成量显著减少, 且呈浓度依赖性, 与溶剂对照组相比, P < 0.05;GbE(10, 50, 100, 150, 200mol/L)可以浓度依赖性的减少LDH释放、MDA的生成量, 增加SOD的生成量。结论 GbE可拮抗TCE引起的人KC损伤。Abstract:Objective To fur ther assess the effects of extracts from the leaves of Ginkgo biloba on Trichloroethy lene (TCE)induced normal human epidermal keratinocytes(NHEK)oxidation insult.Methods Cytotoxicity was determined by netrualred uptake(NRU)and lactate dehydrogenase release(LDH)test, lipid peroxidation of cells was evaluated by malon dialdehyde(MDA)and superoxide dismutase(SOD), respectively.Results Cytotoxicity asays yielded the concentration producing 50% reduction of Netrual Red Uptake(NR50)for TCE was 4 53 mmol/L.Compared with control, treatment of with various concentration of TCE caused a great decrease in cell viability(P < 0.05).A concentration and time dependent release of LDH and a concentration dependentincrease of MDA levels were observed.In contrast, SOD activity was inhibited by concentration dependent mode.Ginkgo biloba 10~200 mg/L dose dependently attenuated the cytotoxic effect of TCE.Ginkgo biloba also inhibited TCE(0.5 mmol/L)induced LDH release, elevation of lipide peroxidation and decline of antioxidant enzyme activities.Conclusion Ginkgo biloba protected NHEK from TCE induced cytotoxicity.
下载: